Changes

**<s>Identify two journals (plan A and plan B) to submit Brandon's study to.</s> Understand their submission requirements. <s>Write up the introduction for the study in LaTeX</s>, formatted and tailored appropriately for each journal.

**<s>Identify two journals (plan A and plan B) to submit Brandon's study to.</s> Understand their submission requirements. <s>Write up the introduction for the study in LaTeX</s>, formatted and tailored appropriately for each journal.

***[[A&A submission requirements]]

***[[A&A submission requirements]]

**Feasability study of SCD-PV

***[[Journal for Obstetric Anesthesia submission requirements]]

**<s>Feasability study of SCD-PV</s>

**<s>Lit review and, if novel</s>, data sharing agreement for Methohexital

**<s>Lit review and, if novel</s>, data sharing agreement for Methohexital

**<s>Narrow down research directions and goals</s>

**<s>Narrow down research directions and goals</s>

**Literature review to refine research aims

**Literature review to refine research aims

**Identify mentors and institutional resources

**<s>Identify mentors and institutional resources</s>

*Identify additional funding sources and apply

*Identify additional funding sources and apply

*Start recruiting a research team

*Start recruiting a research team

==10/2 Summary==

===10/2 Summary===

*E-mailed slides to Nina and followed up on study. It is currently paused due to a hyperbaric PF bupivacaine shortage at OHSU.

*E-mailed slides to Nina and followed up on study. It is currently paused due to a hyperbaric PF bupivacaine shortage at OHSU.

*Texted Brandon about which journals to submit to. 1st choice: A&A, 2nd choice: Journal for Obstetric Anesthesia

*Texted Brandon about which journals to submit to. 1st choice: A&A, 2nd choice: Journal for Obstetric Anesthesia

*Hamilton and I talked about why ketamine causes lasting changes in the brain long after the drug is gone. Is it a change to a gap junction network? '''Is it anti-inflammatory circulating EVs?'''. There are three papers on MedLine related to ketamine and EVs, none of which look at (anti-)inflammatory molecules. '''Do circulating EVs after propofol anesthetic also contain the inflammatory cytokines that are seen after isoflurane?'''

*Hamilton and I talked about why ketamine causes lasting changes in the brain long after the drug is gone. Is it a change to a gap junction network? '''Is it anti-inflammatory circulating EVs?'''. There are three papers on MedLine related to ketamine and EVs, none of which look at (anti-)inflammatory molecules. '''Do circulating EVs after propofol anesthetic also contain the inflammatory cytokines that are seen after isoflurane?'''

==10/6 Summary==

===10/6 Summary===

*Pitched multi-centre trial to Brandon. E-mailed slides in .ppt format to Nina.

*Pitched multi-centre trial to Brandon. E-mailed slides in .ppt format to Nina.

*Met w/ Norm about depression research (Brian Mickey and Grace Lee) and promotion requirements (Harriet Hopf). '''Need to read this paper about ketamine having an active metabolite that sticks around, explaining its sustained antidepressant effects''' ([https://scholar.googleusercontent.com/scholar.bib?q=info:oxmvfF2RMfcJ:scholar.google.com/&output=citation&scisdr=CiBKb9IEEIjgixuUERc:ACTRDVEAAAAAas-SCRfDvqsYSFStZqKmI5mpZP0&scisig=ACTRDVEAAAAAas-SCWl_hrwNxs7LrCdW_sSZWZk&scisf=4&ct=citation&cd=-1&hl=en Nature paper]).

*Met w/ Norm about depression research (Brian Mickey and Grace Lim) and promotion requirements (Harriet Hopf). '''Need to read this paper about ketamine having an active metabolite that sticks around, explaining its sustained antidepressant effects''' ([https://scholar.googleusercontent.com/scholar.bib?q=info:oxmvfF2RMfcJ:scholar.google.com/&output=citation&scisdr=CiBKb9IEEIjgixuUERc:ACTRDVEAAAAAas-SCRfDvqsYSFStZqKmI5mpZP0&scisig=ACTRDVEAAAAAas-SCWl_hrwNxs7LrCdW_sSZWZk&scisf=4&ct=citation&cd=-1&hl=en Nature paper]).

*Lit review of Yohimbine and Naloxone effects on EEG.

*Lit review of Yohimbine and Naloxone effects on EEG.

*Dug through supplement of 2026 A&A paper to find which anesthetics are most selective for which receptors ''in vitro''.

*Dug through supplement of 2026 A&A paper to find which anesthetics are most selective for which receptors ''in vitro''.

*[https://pmc.ncbi.nlm.nih.gov/articles/PMC12998346/ Remimazolam targets]: the theoretical initial target concentration of remimazolam may be 0.1 µg/mL for light sedation, 0.2 µg/mL for moderate sedation, and 0.3 µg/mL for deep sedation in elderly patients (70 years), and 1.5 or 2 times those concentrations in middle-aged (45 years) or young adult (20 years) patients. Dose adjust upwards by 10% in females, down by 20% in obesity or ASA III/IV.

*[https://pmc.ncbi.nlm.nih.gov/articles/PMC12998346/ Remimazolam targets]: the theoretical initial target concentration of remimazolam may be 0.1 µg/mL for light sedation, 0.2 µg/mL for moderate sedation, and 0.3 µg/mL for deep sedation in elderly patients (70 years), and 1.5 or 2 times those concentrations in middle-aged (45 years) or young adult (20 years) patients. Dose adjust upwards by 10% in females, down by 20% in obesity or ASA III/IV.

==10/14 Summary==

===10/14 Summary===

*Attended SandLab meeting.

*Attended SandLab meeting.

*Called Christian (consistently has Wed/Thu off, added me to two IRB protocols after I uploaded CITI competency to ERICA system)

*Called Christian (consistently has Wed/Thu off, added me to two IRB protocols after I uploaded CITI competency to ERICA system)

*COMPASS protocol we do need to consent patients (what is it?)

*COMPASS protocol we do need to consent patients

*Cameron Jacobson added access to local SAND lab web disk.

*Cameron Jacobson added access to local SAND lab web disk.

*GitHub can pull functions to work on data from the web disk. Srdjan added me to the main branch.

*GitHub can pull functions to work on data from the web disk. Srdjan added me to the main branch.

*IARS abstract submission deadline is 12/6 (methohexital, ketamine depression, SCD-PV)

*IARS abstract submission deadline is 12/6 (methohexital, ketamine depression, SCD-PV)

==10/20 Summary==

===10/20 Summary===

*Reached out to Houman re: Methohexital data. Lit review of methoxital going back to 1992.

*Reached out to Houman re: Methohexital data. Lit review of methoxital going back to 1992.

*Figured out software and access: web disk (use ethernet, VPN, or eduroam), GitHub repos, EEGLAB, MatLab, Anaconda

*Figured out software and access: web disk (use ethernet, VPN, or eduroam), GitHub repos, EEGLAB, MatLab, Anaconda

*Submitted NIH Bio Sketch and project summary for possible matching with MSARF student next summer.

*Submitted NIH Bio Sketch and project summary for possible matching with MSARF student next summer.

==10/29 Plan==

===10/29 Summary===

*Requested key for desk and put my name up

*Requested key for desk and put my name up

*Touched base with Grace about retrospective ketamine study

*Touched base with Grace about retrospective ketamine study

**Hamilton is going to start thinking about how to record behavior from multiple wells at once, or how to automate serial well acquisition.

**Hamilton is going to start thinking about how to record behavior from multiple wells at once, or how to automate serial well acquisition.

**I should start by making agar, isolating and plating ''E. coli'' colonies, picking worms, mastering worm husbandry, and acquiring images of WT behaviors.

**I should start by making agar, isolating and plating ''E. coli'' colonies, picking worms, mastering worm husbandry, and acquiring images of WT behaviors.

**Hamilton is doing a screen of ketamine and isoflurane and trying to get genetic lines to test the relevant receptors.

**Hamilton is doing a screen of ketamine and isoflurane and trying to get genetic lines to test the relevant receptors.

===EEG Approach===

===EEG Approach===

*Emergence from different anesthetics in patients with SEEG implants. Can we correlate this with fMRI and scalp EEG or eCOG?

*Can we correlate DECODE data with fMRI or eCOG?

*'''Is emergence just induction backwards? If not, what can that tell you about mechanisms?'''

*Emergence is not just induction backwards. There's neural inertia and hysteresis. What can this tell you about mechanisms of different agents?

===Phylogenetic Approach===

===Phylogenetic Approach===

===Heterodox Hypothesis===

===Heterodox Hypothesis===

*If you can anesthetize single-celled organisms, are circuits irrelevant?

*If you can anesthetize single-celled organisms, are circuits irrelevant?

*Hamilton has a gap junction (network of gap junctions?) and some specific neurons that he think are a conserved mechanism of anesthesia. He wants to test this by doing a screen of a bunch of different anesthetics in WT and KO worms with a behavioral screening assay. He has a behavior rig that basically automatically records swimming speed. He wants to look for the EC50 of various anesthetics and see if that number is shifted with KO or KD of different gap junctions.

*Hamilton has a gap junction (network of gap junctions?) and some specific neurons that he think are a conserved mechanism of anesthesia. He wants to test this by doing a screen of a bunch of different anesthetics in WT and KO worms with a behavioral screening assay. He wants to look for the EC50 of various anesthetics and see if that number is shifted with KO or KD of different gap junctions.

*Worms natively have a blue-light rhodopsin that triggers an aversion response where they turn around and go the opposite direction.

*Worms natively have a blue-light rhodopsin that triggers an aversion response where they turn around and go the opposite direction.

*I am going to '''read up on propofol mechanisms in worms, and gap junctions''' before our next meeting on 10/29.

*'''Read up on gap junctions'''.

==DECODE==

==DECODE==

==Young Blood==

==Young Blood==

*Observational study

*Observational study, talk to Chuck.