1,259
edits
Changes
Jump to navigation
Jump to search
Line 32:
Line 32: − +
→Mechanisms of Anesthesia
*High-throughput screen of anesthetics (and anesthetic reversal agents) in ''C.elegans''. I could work in parallel in a different organism (e.g. yeast, jellyfish, hydra, sea anemones, corral, bacteria, plants, neuronal explants).
*High-throughput screen of anesthetics (and anesthetic reversal agents) in ''C.elegans''. I could work in parallel in a different organism (e.g. yeast, jellyfish, hydra, sea anemones, corral, bacteria, plants, neuronal explants).
*Downsides of using 'weird' organisms is that there are relatively few genetic tools, antibodies, sparse literature, and few experts to consult.
*Downsides of using 'weird' organisms is that there are relatively few genetic tools, antibodies, sparse literature, and few experts to consult.
*'''Has anyone published behavioral assays for these organisms? Detailed protocols for breeding them? Have they already been anesthetized?'''
*Has anyone published behavioral assays for these organisms? Detailed protocols for breeding them? Have they already been anesthetized?
*[https://scholar.googleusercontent.com/scholar.bib?q=info:dK96wvRnWFAJ:scholar.google.com/&output=citation&scisdr=CiBKb9IEEIjgixJyYaI:ACTRDVEAAAAAasZ0eaKpU14jFRxgeJubQZ-AUJs&scisig=ACTRDVEAAAAAasZ0eT0zDfaBVoegO8FEa7bX0-A&scisf=4&ct=citation&cd=-1&hl=en 2026 A&A paper] argues that classifying anesthetics as, say, GABAergic vs. NMDAergic is misleading. Propofol for instance actually has higher potency at the α4β2 nAChR and HCN1 receptors than at the "classic" GABA<sub>A</sub>R receptor. Based on 30 years of in vitro studies (n>330), most anesthetics exhibit similar potency across a range of receptors. Most receptors are non-selective for ligands. What this paper addresses but doesn't answer is whether the receptors are present at the correct locations and necessary to the anesthetic behavioral response.
*[https://scholar.googleusercontent.com/scholar.bib?q=info:dK96wvRnWFAJ:scholar.google.com/&output=citation&scisdr=CiBKb9IEEIjgixJyYaI:ACTRDVEAAAAAasZ0eaKpU14jFRxgeJubQZ-AUJs&scisig=ACTRDVEAAAAAasZ0eT0zDfaBVoegO8FEa7bX0-A&scisf=4&ct=citation&cd=-1&hl=en 2026 A&A paper] argues that classifying anesthetics as, say, GABAergic vs. NMDAergic is misleading. Propofol for instance actually has higher potency at the α4β2 nAChR and HCN1 receptors than at the "classic" GABA<sub>A</sub>R receptor. Based on 30 years of in vitro studies (n>330), most anesthetics exhibit similar potency across a range of receptors. Most receptors are non-selective for ligands. What this paper addresses but doesn't answer is whether the receptors are present at the correct locations and necessary to the anesthetic behavioral response.