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→Non Receptor-Mediated Hypothesis
**Has anyone published behavioral assays for these organisms? How do you make an argument that a slime mold has been anesthetized?
**Has anyone published behavioral assays for these organisms? How do you make an argument that a slime mold has been anesthetized?
===Non Receptor-Mediated Hypothesis===
===Heterodox Hypothesis===
*If you can anesthetize single-celled organisms, are circuits irrelevant?
*Hamilton has a gap junction (network of gap junctions?) and some specific neurons that he think are a conserved mechanism of anesthesia. He wants to test this by doing a screen of a bunch of different anesthetics in WT and KO worms with a behavioral screening assay. He has a behavior rig that basically automatically records swimming speed. He wants to look for the EC50 of various anesthetics and see if that number is shifted with KO or KD of different gap junctions.
*Hamilton has a gap junction (network of gap junctions?) and some specific neurons that he think are a conserved mechanism of anesthesia. He wants to test this by doing a screen of a bunch of different anesthetics in WT and KO worms with a behavioral screening assay. He has a behavior rig that basically automatically records swimming speed. He wants to look for the EC50 of various anesthetics and see if that number is shifted with KO or KD of different gap junctions.
*Worms natively have a blue-light rhodopsin that triggers an aversion response where they turn around and go the opposite direction.
*Worms natively have a blue-light rhodopsin that triggers an aversion response where they turn around and go the opposite direction.