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Line 56: + − *Meeting w/ [https://medicine.utah.edu/faculty/lara-brewer Lara Brewer] re: Respiration.AI+ + + + + + + + + +
→10/29 Plan
==10/29 Plan==
==10/29 Plan==
*Meeting w/ [https://medicine.utah.edu/faculty/lara-brewer Lara Brewer] re: Respiration.AI
*Meeting w/ Kai Kuck re: SCD-PV.
*Meeting w/ Kai Kuck re: SCD-PV.
*Meeting w/ Hamilton re: mechanistic studies in worms
*Meeting w/ Hamilton re: mechanistic studies in worms
**C. elegans has strong cuticle that decreases absorption of lots of drugs. May have to make them eat the drug before hand instead of just bathing them in it. He doesn't know if there's a way to predict which drugs cross the cuticle or validate that a drug in fact made it across.
**Swimming assay (easiest)
**Should be able to build a simple behavioral rig for less than $200
**Recommends connecting with [https://bioscience.utah.edu/faculty/maricq/index.php Andrew Maricq] at UofU who is a worm researcher interested in learning, memory, synapse formation, and neural circuits. He may be able to help get me started with ''C. elegans'' research or collaborate on setting up a screen.
**Hamilton is going to start thinking about how to record behavior from multiple wells at once, or how to automate serial well acquisition.
**I should start by making agar, isolating and plating E.coli colonies, picking worms, mastering worm husbandry, and acquiring images of WT behaviors.
**Eventually we do a screen where we feed the animals different drugs for an hour, then put them in propofol and look for behavioral differences.
**Hamilton is doing a screen of ketamine and isoflurane and trying to get genetic lines to test the relevant receptors.
*Meeting w/ Christian re: research directions
*Meeting w/ Christian re: research directions
*Retrospective ketamine study w/ Grace
*Retrospective ketamine study w/ Grace