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→Genetic Probing of Anesthetic-Receptor Interactions
*'''Where and by what cell types are these receptors expressed in the human brain?'''
*'''Where and by what cell types are these receptors expressed in the human brain?'''
* Hamilton's lab specializes in light-sheet imaging and they have single neuron resolution where they can look at calcium currents (neuronal activity) in real time.
* Hamilton's lab specializes in light-sheet imaging and they have single neuron resolution where they can look at calcium currents (neuronal activity) in real time.
* ''C. elegans'' don't have any histamine signaling natively. You can make animals that express histamine-gated chloride channels to chemogenetically inactivate channel expressing neurons when histamine is given exogenously. '''Look for a GABA<sub>A</sub>R His-Cl worm line.
* ''C. elegans'' don't have any histamine signaling natively. You can make animals that express histamine-gated chloride channels to chemogenetically inactivate channel expressing neurons when histamine is given exogenously.
**There's no GABA<sub>A</sub>R His-Cl worm line.
** He is going to send me some links to worm genetic databases, and some protocols for breeding and behavior.
** He is going to send me some links to worm genetic databases, and some protocols for breeding and behavior.
**GABA is also an important neurotransmitter in [https://www.ncbi.nlm.nih.gov/books/NBK19793/ C. elegans]; however, in contrast to vertebrates where GABA acts at synapses of the central nervous system, in nematodes GABA acts primarily at neuromuscular synapses. Specifically, GABA acts to relax the body muscles during locomotion and foraging and to contract the enteric muscles during defecation.
**GABA is also an important neurotransmitter in [https://www.ncbi.nlm.nih.gov/books/NBK19793/ C. elegans]; however, in contrast to vertebrates where GABA acts at synapses of the central nervous system, in nematodes GABA acts primarily at neuromuscular synapses. Specifically, GABA acts to relax the body muscles during locomotion and foraging and to contract the enteric muscles during defecation. It is located on the post-synaptic side of the neuromuscular junction [https://wormbase.org/species/c_elegans/gene/WBGene00006784#0-9f-10 source]
===EEG Approach===
===EEG Approach===