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→Mechanisms of Anesthesia
*'''Are there pharmacological agents with receptor specificity? When given together with "promiscuous" anesthetics, what happens? For instance, is propofol equally effective when given with a specific HCN1 receptor inhibitor? What about a GABA<sub>A</sub>R inhibitor?'''
*'''Are there pharmacological agents with receptor specificity? When given together with "promiscuous" anesthetics, what happens? For instance, is propofol equally effective when given with a specific HCN1 receptor inhibitor? What about a GABA<sub>A</sub>R inhibitor?'''
*'''Do they cross the blood brain barrier?'''
**'''Search [https://www.ebi.ac.uk/chembl/explore/compound/CHEMBL63354/ ChEMBL]
**'''Search [https://www.ebi.ac.uk/chembl/explore/compound/CHEMBL63354/ ChEMBL]
*'''Are there genetic KO/KD or optogenetic tools available for location-specific inducible receptor knockdowns?'''
*'''Are there genetic KO/KD or optogenetic tools available for location-specific inducible receptor knockdowns?'''
**Is the receptor, its components, or homologues of the gene expressed in different model organisms? What is the sequence similarity?
**'''Is the receptor, its components, or homologues of the gene expressed in different model organisms? What is the sequence similarity with human?'''
**Search [https://flybase.org flybase], [https://www.informatics.jax.org/ MGI], [https://www.wormbase.org/ wormbase], [https://zfin.org/ zebra fish information network], [https://www.yeastgenome.org/ yeast genome database]
**'''Search [https://flybase.org flybase], [https://www.informatics.jax.org/ MGI], [https://www.wormbase.org/ wormbase], [https://zfin.org/ zebra fish information network], [https://www.yeastgenome.org/ yeast genome database]'''
'''*Hamilton has a gap junction (network of gap junctions?) and some specific neurons that he think are a conserved mechanism of anesthesia. He wants to test this by doing a screen of a bunch of different anesthetics in WT and KO worms with a behavioral screening assay. He has a behavior rig that basically automatically records swimming speed. He wants to look for the EC50 of various anesthetics and see if that number is shifted with KO or KD of different gap junctions.
*Hamilton has a gap junction (network of gap junctions?) and some specific neurons that he think are a conserved mechanism of anesthesia. He wants to test this by doing a screen of a bunch of different anesthetics in WT and KO worms with a behavioral screening assay. He has a behavior rig that basically automatically records swimming speed. He wants to look for the EC50 of various anesthetics and see if that number is shifted with KO or KD of different gap junctions.
*Worms natively have a blue-light rhodopsin that triggers an aversion response where they turn around and go the opposite direction.
*Worms natively have a blue-light rhodopsin that triggers an aversion response where they turn around and go the opposite direction.
*'''Next call 10/14.'''
*'''Next call 10/14.'''